si-Fi is a specialized software tool for RNA interference (RNAi) design and off-target prediction. It is built to evaluate long double-stranded RNA constructs for target specificity and gene silencing efficiency. si-Fi also assists in identifying optimal sequence regions suitable for RNAi construct design, offering both tabular and graphical result outputs for easy interpretation.

To use it, simply paste your RNAi trigger sequence and screen for potential targets and off-targets using your local sequence database. Unlike many web-based tools, si-Fi gives you full control over your data by working offline with custom databases. Any multi-FASTA formatted sequence database can be imported in just a few clicks. In addition, Unigene datasets for multiple species are available for download from dedicated online repositories.